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Development of a method for determination of antibiotics in milk using high performance liquid chromatography
Juglová, Zuzana ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
This diploma thesis focuses on optimization and validation of the method for a mixture of tetracycline antibiotics (oxytetracycline, tetracycline) determination using high performance liquid chromatography with a diode array detector (HPLC–DAD). The aim of this thesis was to determine suitability of the system for the determination of these antibiotics in milk. The theoretical part is focused on individual groups of antibiotics and their mechanism of action, resistances and possibilities of use. Next point of this thesis is legislation, which describes permitted amounts of antibiotics in milk. In this study, the HPLC method and validation parameter is described in detail. The experimental part deals with creation and optimization of the method and its validation. Following validation parameters were verified: system suitability, linearity, method accuracy, limit of detection and quantification, robustness. The results obtained indicate mostly suitable ratio of mobile phase of 0,01 M trifluoracetic acid:methanol:acetonitrile = 80:10:10. The wavelength 355 nm was found as the most feasible for this method after evaluating the result. Determination of robustness showed that the method is robust, and the only significant effect on the robustness was observed in column changes.
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Interactions of pharmaceuticals with humic substances in model hydrogels
Fleischhackerová, Claudia ; Krouská, Jitka (referee) ; Klučáková, Martina (advisor)
This bachelor's thesis is focused on the study of drug diffusion in humic acid hydrogels. The instantaneous surface source diffusion method was used for this study. This method was based on the observation of emerging diffusion profiles of drugs which diffused in the humic gel at certain time intervals. Then, this method was used to determine the diffusion coefficients of the drugs used in the gels. The main aim of the experimental part was to study the influence of the interaction of drugs with humic acids that diffused through the humic gel. Two drugs were used as a diffusing medium, i.e. ibuprofen and tetracycline. They are pharmaceuticals, the consumption has been increasing in humans and animals in recent years, that's why they are often detected in the environment. The concentration profile of these drugs in humic acid hydrogels was determined by UV–VIS spectrometry.
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Development of a method for determination of antibiotics in milk using high performance liquid chromatography
Juglová, Zuzana ; Pořízka, Jaromír (referee) ; Diviš, Pavel (advisor)
This diploma thesis focuses on optimization and validation of the method for a mixture of tetracycline antibiotics (oxytetracycline, tetracycline) determination using high performance liquid chromatography with a diode array detector (HPLC–DAD). The aim of this thesis was to determine suitability of the system for the determination of these antibiotics in milk. The theoretical part is focused on individual groups of antibiotics and their mechanism of action, resistances and possibilities of use. Next point of this thesis is legislation, which describes permitted amounts of antibiotics in milk. In this study, the HPLC method and validation parameter is described in detail. The experimental part deals with creation and optimization of the method and its validation. Following validation parameters were verified: system suitability, linearity, method accuracy, limit of detection and quantification, robustness. The results obtained indicate mostly suitable ratio of mobile phase of 0,01 M trifluoracetic acid:methanol:acetonitrile = 80:10:10. The wavelength 355 nm was found as the most feasible for this method after evaluating the result. Determination of robustness showed that the method is robust, and the only significant effect on the robustness was observed in column changes.
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